2. Signaling Pathways
  3. Cell Cycle/DNA Damage
  4. DNA/RNA Synthesis

DNA/RNA Synthesis

DNA/RNA Synthesis

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RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

DNA/RNA Synthesis Isoform Specific Products:

DNA/RNA Synthesis Isoform Comparison
Cat. No. Product Name Effect Purity Chemical Structure
  • HY-138604
    5'-O-DMT-N6-Me-2'-dA
    		Activator 98.00%
    5'-O-DMT-N6-Me-2'-dA is a nucleoside with protective and modification effects.
    5'-O-DMT-N6-Me-2'-dA
  • HY-W042357S4
    Ac-rC Phosphoramidite-13C9
    Ac-rC Phosphoramidite-13C9 is 13C-labeled Ac-rC Phosphoramidite (HY-W042357). Ac-rC Phosphoramidite is used for the oligoribonucleotide phosphorodithioate modification (PS2-RNA).
    Ac-rC Phosphoramidite-<sup>13</sup>C<sub>9</sub>
  • HY-129046B
    RNase A, Bovine Pancreas DNase & Protease Free
    		Inhibitor
    RNase A (Bovine pancreatic RNase) is a widely used Endonuclease in DNA purification by specifically hydrolyzing cytosine or uracil residues of RNA. RNase A degrades the RNA in the RNA/DNA duplex. RNase A catalyses the breakdown of 3',5'-phosphodiester linkages of single stranded RNA. RNase A family members in organisms are tightly involved in various physiological and pathological processes including cell growth and development, proliferation, differentiation and migration. Dysregulation of RNase A activity or expression level is closely related to pancreatic, ovarian, bladder and thyroid cancer. RNase A has tumor cell-killing ability. RNase A, Bovine Pancreas (DNase & Protease Free) is RNase A derived from bovine pancreas and does not contain DNase or protease.
    RNase A, Bovine Pancreas DNase & Protease Free
  • HY-126258
    IMP-1700
    		Inhibitor
    IMP-1700 is a potent DNA damage potentiator and antibacterial agent. IMP-1700 inhibits the bacterial SOS response to DNA damage. IMP-1700 potently sensitizes MRSA to Ciprofloxacin (HY-B0356) with an EC50 of 5.9 nM. IMP-1700 inhibits the growth of E. coli K-12 BW25113, S. aureus SH1000 and MRSA USA300 JE2.
    IMP-1700
  • HY-139099A
    Diguanosine 5′-triphosphate lithium
    		Modulator
    Diguanosine 5′-triphosphate (Gp3G) lithium is a dinucleoside triphosphates. Diguanosine 5′-triphosphate lithium also is a virus-specific oligonucleotide. Diguanosine 5′-triphosphate lithium is needed for the synthesis of RNA during the transcription process.
    Diguanosine 5′-triphosphate lithium
  • HY-160983
    PHYLPA-8
    		Inhibitor
    PHYLPA-8 is an inhibitor for DNA polymerase-α, which exhibits weak inhibitory activity for the stereochemistry of the fatty acid moiety and the cyclic phosphate.
    PHYLPA-8
  • HY-158662
    3-Me-5-OMe-UTP sodium
    3-Me-5-OMe-UTP sodium is a labeled modified deoxyoligonucleotide (dNTP) that can release pyrophosphate to produce fluorescence and has special applications in gene synthesis and sequencing.
    3-Me-5-OMe-UTP sodium
  • HY-113659
    3',5'-O-Bis(isobutiryl)-4'-azido-2'-β-C-methyl-2'-deoxycytidine
    3',5'-O-Bis(isobutyryl)-4'-azido-2'-β-C-methyl-2'-deoxycytidine functions as an NS5B inhibitor of RNA polymerase, and is utilized in the treatment of HCV infection.
    3',5'-O-Bis(isobutiryl)-4'-azido-2'-β-C-methyl-2'-deoxycytidine
  • HY-182817
    Len-604
    		Inhibitor
    Len-604 is a FGFR inhibitor with IC50 values of 9.71 nM, 9.93 nM, 29.80 nM and 14.48 nM against FGFR4, FGFR1, FGFR2 and FGFR3, respectively. Len-604 reduces the phosphorylation levels of FGFR4 and ERK, and induces DNA damage and apoptosis in cancer cells. Len-604 is applicable to research related to liver cancer.
    Len-604
  • HY-161161
    aTAG 2139-NEG
    aTAG 2139-NEG (Compound 23) binds to MTH1 and has no degradative activity with a Ki value of 2.0 nM. aTAG 2139-NEG serves as aTAG 2139 negative control.
    aTAG 2139-NEG
  • HY-108875
    Erythromycin stearate
    		Inhibitor
    Erythromycin stearate is a macrolide antibiotic produced by actinomycete Streptomyces erythreus with a broad spectrum of antimicrobial activity. Erythromycin stearate binds to bacterial 50S ribosomal subunits and inhibits RNA-dependent protein synthesis by blockage of transpeptidation and/or translocation reactions, without affecting synthesis of nucleic acid[1][2]. Erythromycin stearate also exhibits antitumor and neuroprotective effect in different fields of research[3][4].
    Erythromycin stearate
  • HY-117171
    Proquinazid
    		Modulator
    Proquinazid is a Bcl-2 and PCNA modulator, oxidative stress inducer, genotoxin and apoptosis inducer. Proquinazid causes DNA damage and also triggers apoptosis by regulating pro-apoptotic and anti-apoptotic proteins.
    Proquinazid
  • HY-132974
    Aminoflavone
    		Inhibitor
    Aminoflavone is an anti-tumor agent. Aminoflavone inhibits the expression of ITGA6/SOX2 by activating the AhR-miR-125b-2-3p axis, thereby targeting breast cancer stem cells. Aminoflavone induces an increase in intracellular ROS, increases the level of oxidative DNA damage marker 8-oxodG and DNA-protein cross-links. Aminoflavone causes S-phase arrest, activates caspase-3/8/9 and induces apoptosis (apoptosis). Aminoflavone inhibits HIF-1α expression in a manner independent of AhR. Aminoflavone can be used for the study of breast cancer.
    Aminoflavone
  • HY-131561
    Ara-UTP
    Ara-UTP is a sugar-modified nucleotide triphosphate that deceives RNA polymerases and blocks chain extension.
    Ara-UTP
  • HY-150239
    BAY-364
    		Inhibitor
    BAY-364 (BAY-299N) is an inhibitor of the second bromine domain in TAF1. BAY-364 inhibits the TAF1 of Kasumi-1 cells, CD34+ cells and K562 cells with IC50 values of 1.0 µM, 10.4 µM and 10.0 µM respectively.
    BAY-364
  • HY-181994
    RNAP-IN-1
    		Inhibitor
    RNAP-IN-1 is a ribonucleic acid polymerase (RNAP) inhibitor, with an IC50 of 0.95 μM against E. coli RNAP. RNAP-IN-1 can be used in studies of bacterial infections.
    RNAP-IN-1
  • HY-B0147S
    Pefloxacin-d5
    		Inhibitor 98.33%
    Pefloxacin-d5 (Pefloxacinium-d5) is the deuterium labeled Pefloxacin (HY-B0147). Pefloxacin (Pefloxacinium) is a broad spectrum antibiotic. Pefloxacin blocks DNA replication by inhibiting DNA gyrase. Pefloxacin inhibits DNA relaxation catalyzed by topoisomerase I with an IC50 of 45 μg/mL. Pefloxacin exhibits antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, and Bacteroides fragilis with MIC90s of 0.12, 4, and 16 mg/L, respectively. Pefloxacin has anti-Plasmodium yoelii infection activity. Pefloxacin increase UVA-induced edema and immunesuppression. Pefloxacin can be used for infection studies.
    Pefloxacin-d<sub>5</sub>
  • HY-157611
    DHX9-IN-17
    		Inhibitor
    DHX9-IN-17 (186) is a RNA helicase DHX9 inhibitor, with an EC50 of 0.161 μM in DHX9 cellular target engagement. Used in cancer research.
    DHX9-IN-17
  • HY-138588
    7-TFA-ap-7-Deaza-ddA
    7-TFA-ap-7-Deaza-ddA (compound 19c, US20060281100A1), a nucleotide derivative, can be used in the synthesis of thiotriphosphate nucleotide dye terminators which can be used in DNA sequencing reactions.
    7-TFA-ap-7-Deaza-ddA
  • HY-13689G
    Go 6983 (GMP)
    		Activator
    Go 6983 GMP is Go 6983 (HY-13689) produced by using GMP guidelines. GMP small molecules works appropriately as an auxiliary reagent for cell therapy manufacture. Go 6983 is a dual inhibitor targeting Suv39h1/2 (KMT1A/KMT1B) and PKC, as well as a transcriptional activator capable of inducing DNA hypomethylation. Go 6983 stimulates the transcription of Prdm14 by reducing Suv39h1/2 protein levels, decreasing histone modifications in the Prdm14 promoter region, and increasing the recruitment of RNA polymerase II. Go 6983 induces genome-wide DNA hypomethylation by inhibiting de novo methyltransferase expression and increasing Tet1/Tet2 levels, thereby promoting self-renewal and pluripotency maintenance of stem cells. Meanwhile, Go 6983 can block PKC-mediated signaling pathways to reduce the expression of EMT-related genes and eliminate the upregulation of antioxidant genes downstream of NRF2. Go 6983 is mainly used in mechanism studies related to myocardial ischemia/reperfusion injury.
    Go 6983 (GMP)
Cat. No. Product Name / Synonyms Application Reactivity
DNA/RNA Synthesis Isoform Comparison

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